Enzyme activity Essay

Purpose The purpose of this laboratory is to try for enzyme action mechanism, look at enzyme specificity, and how temperature affects enzyme activity.Time need to perform this lab approximately 3 hoursPreparation runner Read the lab in its entirety TWICE before you begin. You will perform the experiment, write your lab hide and overwhelm the answers to the additional 4 questions indoors the text for full credit on this experiment.Materials3% hydrogen bleacha household blenderpotatoes (3 medium coat potatoes, that ar peeled and quartered) work stoppage water8 test subway systems8 transfer pipetssugar packetssalta small saucepana refrigerator with a freezerstove4 clear plastic cupstongs (to remove test tubes from boiling water)4 coffee cupscheesecloth to filter the potato extracta wax pencil or Sharpie markera rulerscale or balance250 ml graduated cylinderoven script or tongsPreparation flavour 1 Research (online) the reaction between catalase andhydrogen peroxide and be f amiliar with the reactants, products, and enzyme. You will include the overall reaction in your lab report for this experiment. We will measure enzyme activity by beat the height of the talks produced.Questions1.Given your research, why are we measuring the height of the bubbles produced to indicate how much enzyme activity has occurred? 2.What product are we specifically observing being produced?Preparation pace 21.Prepare the potato extract (catalase) using the following technique peel 3 medium size potatoes and cut them into quartersadd the potatoes to the blender with 250 mls of tap water to cover the potatoes and blend on high until the potatoes are pureed (blended to a thick liquid or paste) rate a cheesecloth over a container and use the cheesecloth to obtain the liquid portion (only) of your potato puree. You can squeeze the cheesecloth in the end to obtain the last(a) portions of the liquid from the puree. 1.Make a 1% sucrose solution using the method describe in your osmosis lab. 2.Make a 1% salt solution by measuring 1 gram of salt using your scale or balance, add this to your graduated cylinder and whence fill to the 100ml mark. You can then transfer this compartmentalisation to a small saucepan, heat and stir until the mixture is dissolved.ProcedureExperiment 1 Testing for Enzymatic ActivityFor this experiment, you will need4 test tubesrulerwater1% sucrose (sugar) solution1% salt solution3% hydrogen peroxidecatalase (the liquid portion of your potato extract)4 clear plastic cupswax penciltransfer pipetsStep 1 Add 3mls of the following solutions to a labeled test tube using a fresh transfer pipet with each new solutiontest tube 1 tap watertest tube 2 1% sucrose solutiontest tube 3 1% salt solutiontest tube 4 3% hydrogen peroxideQuestions1.Why is it outstanding to use a fresh transfer pipet for each new solution? Step 2 Add 2 mls of the catalase solution (liquid portion of the potato extract) to each test tube and place into a plastic cup to keep it upright. Pick up each test tube, tap the bottom/ spin around the contents and then using your ruler record in Table 1 the bubble height (if any) that is produced. Remember to include this table (with a strong title) in your lab report.Experiment 2- Testing the Effect of Temperature on Enzyme ActivityFor this experiment, you will need4 test tubesruler3% hydrogen peroxidecatalase (the liquid portion of your potato extract)4 coffee cupswax penciltransfer pipetsrefrigerator w/ freezerstovesaucepantap wateroven mitt or tongsStep 1 Label the test tubes 1, 2, 3 and 4, where test tube 1 represents the sample placed on the counter at room temperature, test tube 2 represents the tube placed in the refrigerator, test tube 3 represents the tube placed in the freezer, and test tube 4 represents the sample exposed to boiling water. You will expose catalase to each of these four conditions.Questions4.Before you begin, predict under which treatment you expect to see the most enzyme activ ity and explain why you think that will happen. Step 2 Place a saucepan with tap water on a burner and bring to a boil.Step 3 Use the thermometer to identify and record the temperature for room temperature, in your refrigerator, in your freezer, and then research the temperature of boiling water (do not take this temperature) and record them in Table 2.Step 4 Place the test tubes into separate coffee cups to maintain the upward po rallyion. Add 2 mls of the catalase solution to each of the test tubes and then place tubes1, 2, and 3 in the conditions described above. For test tube 4, fill the coffee mug half full of boiling water and then place test tube 4 in this container. Do not place the test tube into the pan of boiling water.Step 5 Allow the tubes to sit for 5 minutes, then remove the tubes from the conditions mentioned above (note be careful to use an oven mitt or tongs to remove the test tube exposed to boiling water from the coffee cup).Step 6 Then add 3 mls of 3% hydrogen p eroxide, swirl all tubes, and measure the bubbles produced by measuring bubble height (if any are produced). Record this data in Table 2.ResultsPlease include your results in two tables that are clearly labeled and realize columns for the contents of each test tube, the treatment if applicable (experiment 2) and the height of the bubbles produced (if any).ConclusionsFor this portion, include the results obtained and an interpretation of the results. Also state whether the results were expected or not and, if the results are unexpected, include possible reasons for this and what your next step should be. Remember to also include your answers to the 4 Questions embedded in the experiment above for full credit.Submission Create your lab report using word-processing software, such as Microsoft Word and save it to your computer as a .doc, .docx, or .rtf file. To submit the report, choose the link titled, Lab 6 Enzymes, above. Use the Browse My Computer button in the Attach File area to attach your document. Be sure to complete your submission by choosing the take on button at the bottom of the screen.